Nanosomes carrying mycosynthesized silver nanoparticles as a drug delivery tool against MDR Salmonella typhi BTCB170

Mehwish Iqtedar; Nimra illyas; Mehral Aslam; Roheena Abdullah; Afshan Kaleem

doi:10.62063/ecb-61

Authors

Mehwish Iqtedar Lahore College for Women University
Nimra illyas Lahore College for Women University
Mehral Aslam Lahore College for Women University https://orcid.org/0009-0000-4298-3998
Roheena Abdullah Lahore College for Women University https://orcid.org/0000-0003-3932-6297
Afshan Kaleem Lahore College for Women University https://orcid.org/0000-0002-3972-0051

DOI:

https://doi.org/10.62063/ecb-61

Keywords:

Antibacterial activity, (AgNPs) Silver-nanoparticles, liposomes, Salmonella typhi

Abstract

New regimes of treatments and delivery systems are of great need today because of the increasing emergence of antimicrobial resistance among pathogens. In this study, initially liposomes were prepared comprising mycosynthesized silver nanoparticles in combination with meropenem. As a result of this combination, nanosomes were designed. In vitro analysis of the prepared formulation was done to check its effectiveness against MDR Salmonella typhi. Extracellular synthesis of (AgNPs) silver-nanoparticles through Aspergillus fumigatus BTCB01 was conducted. The particles were then characterized through by a zeta sizer, and they had particle size (299.6 nm), zeta potential (-2.64 mV) with a polydispersity value (0.4). Different liposomal formulations were prepared and characterized, each with different in size i.e. control liposomes had 318.7 nm, drug-encapsulated liposomes had 237.4 nm, silver-nanoparticles incorporated liposomes had 320.3 nm and Silver-nanoparticles incorporated drug liposomes had 215.3 nm size with different zeta potential and polydispersity index for each, i.e. -45.8 (PDI:0.3), -41.3 (PDI:0.3), -42.5 (PDI:0.4) and -39.6 mV (PDI:0.3), respectively, signifying a good stabile and homogeneous solution. Meropenem encapsulated liposomes had (94%) encapsulation efficiency, whereas Silver-nanoparticles incorporated drug liposomes had (86%) encapsulation efficiency. The release study of drugs through dialysis membranes graded in order of drug encapsulated liposomes ˃ Silver-nanoparticles incorporated drug liposomes ˃ control liposomes. In vitro analysis revealed that the antibacterial assay through the disk diffusion method revealed that drug-encapsulated liposomes showed a 1-fold increase in activity as compared to those which were incorporated with the silver nanoparticles. Conclusively, the addition of silver nanoparticles did not affect the stability of liposomes; however, they affected the encapsulation efficiency and drug release property of the delivery system.

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