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DOI DOI: 10.62063/ecb-84

Re-evaluation of population-level associations between DBP gene polymorphisms and the COVID-19 prevalence and mortality: a cross sectional analysis, including pre- and post-vaccination periods

Ahsen Pektas1, Ilknur Tunc1, Buse Aslan1, Nermin Akcali1, Lutfiye Karcıoglu Batur1
  1. 1 Biruni University

Abstract

This study aimed to investigate the correlation between vitamin D-binding protein (DBP) gene polymorphisms at rs4588 and rs7041 loci and the prevalence and mortality rates of the coronavirus disease 2019 (COVID-19) up to 2022. In addition, the study sought to conduct a current situation analysis regarding vaccination among the populations of 10 countries, including Türkiye. The study examined the relationships between the COVID-19 prevalence and mortality rates and the frequencies of different genotypes according to rs7041 and rs4588 loci, which were collected from the literature. Total number of the COVID-19 cases and mortality rate because of SARS-CoV-2 infection before and after vaccination, and the vaccine data, including all types of vaccines, were collected from the World Health Organization coronavirus dashboard in 2022. The data from China, Japan, Nigeria, Kenya, Mexico, Italy, Türkiye, Finland, Germany, and Czechia was included. The mortality rates in China, Nigeria, Mexico, and Italy decreased significantly after the vaccination (P < 0.05), while the prevalence of COVID-19 after the vaccination was not affected in all the countries included (P > 0.05). It was observed that the higher the frequency of GG and GT genotypes of rs7041, the higher the prevalence of COVID19 (P = 0.02 and P = 0.01, respectively), but only frequency of GT genotype was directly associated with increasing mortality rates in all countries during pre-vaccination period (P < 0.01). There was a significant inverse relationship between the frequency of TT genotype of rs7041 and the prevalence of COVID-19 (P = 0.02), but the relationship with mortality rate was not significant in all countries (P > 0.05). No significant correlation was found between the frequencies of AA, AC and CC genotypes of rs4588, and the COVID-19 prevalence and mortality rates in all countries (P > 0.05). Our findings suggest that the vaccination did not change the prevalence of COVID-19 but decreased mortality rates in four of the 10 countries studied. Significant correlations were observed in the frequencies of rs7041 single nucleotide polymorphism (SNP) with the COVID-19 prevalence and mortality rates, while no correlation was observed in those of rs4588 SNP.

How to Cite

Pektas, A., Tunc, I., Aslan, B., Akcali, N., & Karcıoglu Batur, L. (2026). Re-evaluation of population-level associations between DBP gene polymorphisms and the COVID-19 prevalence and mortality: a cross sectional analysis, including pre- and post-vaccination periods. The European Chemistry and Biotechnology Journal, (6). https://doi.org/10.62063/ecb-84
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References

  1. Aleebrahi̇m-Dehkordi̇, E., Deravi̇, N., Yaghoobpoor, S., Hooshyar D, & Rafieian-Kopaei M. (2021). The roles of vitamin D in increasing the body’s immunity and reducing injuries due to viral infections: with an emphasis on its possible role in SARS-CoV-2 (COVID-19). Current Pharmaceutical Design, 27(44), 4452–4463. https://doi.org/10.2174/1381612827666210608145236
  2. Al-Kassmy, J., Pedersen, J., & Kobi̇nger, G. (2020). Vaccine candidates against coronavirus infections. Where does COVID-19 stand? Vi̇rus, 12(8), 861. https://doi.org/10.3390/v12080861
  3. Bi̇kle, David D, & Schwartz, J. (2019). Vitamin D binding protein, total and free vitamin D levels in different physiological and pathophysiological conditions. Frontiers in Endocrinology, 10, 317. https://doi.org/10.3389/fendo.2019.00317
  4. Boucher, B. J. (2020). Vitamin D status as a predictor of Covid-19 risk in black, Asian and other ethnic minority groups in the UK. Diabetes/Metabolism Research and Reviews, 36(8), e3375. https://doi.org/10.1002/dmrr.3375
  5. Cantorna, M. T., Snyder, L., Li̇n, Y. D., & Yang, L. (2015). Vitamin D and 1, 25(OH)2D regulation of T cells. Nutrition, 7(4), 3011–3021. https://doi.org/10.3390/nu7043011
  6. Durmuş, B., Yurtkoru, E. S., & Çinko, M. (2013). Sosyal Bilimlerde Spss’le Veri Analizi [Data Analysis with SPSS in Social Sciences], 5th ed. İstanbul, Türkiye: Beta Yayıncılık.
  7. Enlund-Cerullo, M., Koljonen, L., Holmlund-Suila, E., et al. (2019). Genetic variation of the vitamin D binding protein affects vitamin D status and response to supplementation in infants. Journal of Clinical Endocrinology & Metabolism, 104(11), 5483–5498. https://doi.org/10.1210/jc.2019-00630
  8. Haynes, B. F., Corey, L., Fernandes, P., Gilbert, P. B., Hotez, P. J., Rao, S., & Arvin, A. (2020). Prospects for a safe COVID-19 vaccine. Science Translational Medicine, 12(568), eabe0948. https://doi.org/10.1126/scitranslmed.abe0948
  9. Ili̇e, P. C., Stefanescu, S., & Smith, L. (2020). The role of vitamin D in the prevention of coronavirus disease 2019 infection and mortality. Aging Clinical and Experimental Research, 32(7), 1195–1198. https://doi.org/10.1007/s40520-020-01570-8
  10. Jayawardena, R., Jeyakumar, D. T., Francis, T. V., & Misra, A. (2021). Impact of the vitamin D deficiency on COVID-19 infection and mortality in Asian countries. Diabetes and Metabolic Syndrome, 15(3), 757–764. https://doi.org/10.1016/j.dsx.2021.03.006
  11. Jones, P., Lucock, M., Chaplin, G., Jablonski, N. G., Veysey, M., Scarlett, C., & Beckett, E. (2020). Distribution of variants in multiple vitamin D-related loci (DHCR7/NADSYN1, GC, CYP2R1, CYP11A1, CYP24A1, VDR, RXRα and RXRγ) vary between European, East-Asian and Sub-Saharan African-ancestry populations. Genes & Nutrition, 15(1), 5. https://doi.org/10.1186/s12263-020-00675-1
  12. Karcioğlu Batur, L., & Çöklü, Z. H. (2022a). Avrupa ülkelerinin D vitamini ortalama düzeyleri ile Covıd-19 vaka ve mortalite oranları arasındaki ilişki. Anatolian Clinical Journal of Medical Sciences, 27(2), 127–134. https://doi.org/10.21673/anadoluklin.1019034
  13. Batur, L. K., & Koç, S. (2022b). Association between vitamin D status and secondary infections in patients with severe COVID-19 admitted in the intensive care unit of a tertiary-level hospital in Turkey. Diagnostics, 13(1), 59. https://doi.org/10.3390/diagnostics13010059
  14. Karcioglu Batur, L., & Hekim, N. (2021). The role of DBP gene polymorphisms in the prevalence of new coronavirus disease 2019 infection and mortality rate. Journal of Medical Virology, 93(3), 1409–1413. https://doi.org/10.1002/jmv.26409
  15. Karcioglu Batur, L., Özaydın, A., Mavi̇ş, M. E., Gürsu, G. G., Harbige, L., & Hekim, N. (2021). Vitamin-D binding protein gene polymorphisms and serum 25-hydroxyvitamin-d in a Turkish population. Metaboli̇tes, 11(10), 696. https://doi.org/10.3390/metabo11100696
  16. Khanna, R., Nandy, D., & Senapati, S. (2019). Systematic review and meta-analysis to establish the association of common genetic variations in vitamin D binding protein with chronic obstructive pulmonary disease. Frontiers in Genetics, 10, 413. https://doi.org/10.3389/fgene.2019.00413
  17. Lu, H., Stratton C. W., & Tang, Y. W. (2020). Outbreak of pneumonia of unknown etiology in Wuhan, China: the mystery and the miracle. Journal of Medical Virology, 92(4), 401–402. https://doi.org/10.1002/jmv.25678
  18. Lu, L., Xi̇ong, W., Mu, J., Zhang, Q., Zou, L., & Zhou, D. (2021). The potential neurological effect of the COVID-19 vaccines: a review. Acta Neurologica Scandinavica, 144(1), 3–12. https://doi.org/10.1111/ane.13417
  19. Luri̇e, N., Savi̇lle, M., Hatchett, R, & Halton, J. (2020). Developing COVID-19 vaccines at pandemic speed. New England Journal of Medicine, 382(21), 1969–1973. https://doi.org/10.1056/NEJMp2005630
  20. Pleva, L., Kovarova, P., Faldynova, L, et al. (2015). The rs1803274 polymorphism of the BCHE gene is associated with an increased risk of coronary in-stent restenosis. BMC Cardiovascular Disorders, 15, 135. https://doi.org/10.1186/s12872-015-0128-8
  21. Polack, F. P., Thomas, S. J., Ki̇tchi̇n, N., Absalon, J., Gurtman, A., Lockhart, S., & Gruber, W. C. (2020). Safety and efficacy of the BNT162b2 mRNACOVID-19 vaccine. New England Journal of Medicine, 383(27), 2603–2615. https://doi.org/10.1056/NEJMoa2034577
  22. Rivera-Paredez, B., Hidalgo-Bravo, A., de la Cruz-Montoya, A., et al. (2020). Association between vitamin D deficiency and common variants of vitamin D binding protein gene among Mexican Mestizo and indigenous postmenopausal women. Journal of Endocrinological Investigation, 43(7), 935–946. https://doi.org/10.1007/s40618-019-01177-5
  23. Rozmus, D., Plominski, J., Augustyn, K. & Cieślińska, A. (2022). rs7041 and rs4588 polymorphisms in vitamin D binding protein gene (VDBP) and the risk of diseases. International Journal of Molecular Sci̇ence, 23(2), 933. https://doi.org/10.3390/ijms23020933
  24. Terock, J., Hannemann, A., Van der Auwera, S., et al. (2020). Posttraumatic stress disorder is associated with reduced vitamin D levels and functional polymorphisms of the vitamin D binding-protein in a population-based sample. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 96, 109760. https://doi.org/10.1016/j.pnpbp.2019.109760
  25. Vi̇ti̇ello, A., Ferrara, F., Troi̇ano, V., & La Porta, R. (2021). COVID-19 vaccines and decreased transmission of SARS-CoV-2. Inflammopharmacology, 29(5), 1357–1360. https://doi.org/10.1007/s10787-021-00811-0
  26. Voysey, M., Clemens, S. A. C., Madhi̇, S. A., Weckx, L. Y., Folegatti, P. M., Aley, P.K., & Bijker, E. (2021). Safety and efficacy of the ChAdOx1 nCoV-19 vaccine (AZD1222) against SARS-CoV-2: an interim analysis of four randomised controlled trials in Brazil, South Africa, and the UK. The Lancet, 397(10269), 99–111. https://doi.org/10.1016/S0140-6736(20)32661-1
  27. Wi̇ersi̇nga, W. J., Rhodes, A., Cheng, A. C., Peacock, S. J., & Prescott, H. C. (2020). Pathophysiology, transmission, diagnosis, and treatment of coronavirus disease 2019 (COVID-19): a review. Journal of the American Medical Association, 324(8), 782–793. https://doi.org/10.1001/jama.2020.12839
  28. Zachari̇oudaki̇, M., Messari̇taki̇s, I., & Galanaki̇s, E. (2021). Vitamin D receptor, vitamin D binding protein and CYP27B1 single nucleotide polymorphisms and susceptibility to viral infections in infants. Scientific Reports, 11(1), 13835. https://doi.org/10.1038/s41598-021-93214-9
  29. Zhou, L., Zhang, X., Chen, X., et al. (2012). GC. International Journal of Clinical and Experimental Medicine, 5(1), 72–79. https://doi.org/10.1016/j.ijcem.2012.01.012